Proteomic, genomics and post genomics could benefit from materials science and nanotechnology, which may offer innovative solutions to some of the open questions, i.e.:
- low cost and high throughput gene or protein expression profiling methods;
- high reproducible methods to analyze specific antigen expression patterns and protein interactions in cells and sera at very low concentration;
- perform genotyping and haplotyping by using probes detectable on a single DNA strand at distances as low as 10 nucleotides apart;
- mass selection and mass characterization of macromolecules by using nano/molecular sieves
Recent advances in materials science and nanotechnologies have been emerging with precise control over material chemical, physical and morphological properties and with the enhanced capability to immobilize into/within synthetic platforms molecular signal for particular moieties detection.
The objective of the research will be realization of new biosensor for a fast, high throughput and multiple detection of biomolecules by building into 3D platforms biomolecules recognizing moieties.
3D integrated platforms for high throughput and multiple detection
Molecular recognition is one of the key aspects in cell-cell and cell-environment communication. As cells may sense individual and diverse molecules, the design of novel biosensors may be inspired by the molecular interactions occurring at the cell membrane level.
Exploiting such strategy may allow the creation of sensing devices characterized by high sensitivity and throughput. Recent advances in materials science have provided innovative processing procedures which allow a tight control over material chemical, physical and morphological properties.
Moreover, nanotechnologies have proved the feasibility of encoding signals at a molecular level and with high spatial resolution onto material platforms. This body of knowledge may be tremendously helpful in the field of proteomic, genomics and post genomics, offering solutions to tackle important practical aspects: cost reduction; improve the consistency of the analyses; increase data throughput; enhance spatial resolution. For instance the combination of materials science and nanotechnology has allowed the design and the realization of micro-devices to detect complex array of molecules of diagnostic interest.
Research activities that will be undertaken during this project concern the determination of innovative and specifically screened molecules to be used as sensor moieties toward customized biomolecules and the development of technologies for the integration of such molecular sensors into scaffolding materials especially designed for their effective presentations.